Baylor St. Luke's Medical Center
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Test Information - Anaerobic Culture
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Test Information - Anaerobic Culture

                                                                                                                                                                                                                                                                                                                                                                                                               
NAME OF TESTAnaerobic         Culture
Where Test is PerformedMain         Laboratory
Department NameMicrobiology
Department Location1st         Floor, Rm P120
How to ScheduleOrder         in HBOC
Test         AvailabilitySpecimens         accepted 24 hours, 7 days a week, however, submit between         7:00 and 22:30 whenever possible.

Culture results: Preliminary after         48 hours; Final after 3-5 days.

       
Stat         RequestNo
Additional         InformationDoes not include Routine         Culture or Gram Stain (these must be ordered separately).
SPECIMEN REQUIREMENTS
Specimen         TypeWound,         Abscess, Drainage, Tissue, Body Fluids, Surgical         Specimens

NOT         ACCEPTABLE: Specimens not collected in anaerobic         transport device, cervical or vaginal specimens,         expectorated sputum, bronchoscopic specimens above the         oropharynx, gastric and small bowel contents, large bowel         contents and feces (except for Clostridium Botulinum),         voided and catheterized urine, urethral specimens,         ileostomy and colostomy specimens, specimens from the         oropharynx or nasopharynx, heart valve

       
Specimen         AmountSwab, tissue,         fluid, exudate, any amount
ContainerAspirates in         sterile syringes and tissues are preferable. Anaerobic         transport device (Anaswab or Port-a-cult) or sterile         container only for tissues and large quantities of body         fluids if submitted immediately after collection.
CollectionRefer to         collection instructions for applicable specimen type when         appropriate. Wear gloves during all collection procedures         to avoid specimen contamination.

Surface wounds:         Clean the orifice of sinus tract, surrounding skin or         exterior of abscess with antiseptic to reduce         contamination. Open the lesion and express pus if         possible.

       

Deep Closed Wounds:         Decontaminate the skin surface. Obtain the specimen as         close to the base of active infection as possible.         Aspirate material using a needle and syringe. Expel any         air from the syringe. Remove the needle and cap the         syringe with plastic cap.

       

Collection using anaerobic         transport tube: If the specimen is obtained by swab, hold         the tube upright, unscrew the cap and insert the swab         into the gel. Break off the swab, leaving at         least 1 inch above the top of the gel, and         secure cap tightly. Small pieces of tissue may also be         placed in the tube on top of the gel surface. DO         NOT imbed tissue into the gel. Place larger         pieces of tissue in a sterile container and deliver         immediately to the laboratory.

       
LabelingRefer to         Pathology- 'Collection of Specimen'
TransportDO         NOT REFRIGERATE! Do not use pneumatic         tube system for transport to lab. Transport         ASAP. If transport will be delayed > 30 minutes after collection, allow         specimen to remain at room temperature. For tissue specimens, transport immediately if         unable to use anaerobic transport tube.
To         CancelCancel on         HBOC and discard barcode label.
HBOC         DowntimeUse a         Microbiology Test Order Form. Check Cult-Anaerobic.         Complete all requested information and indicate specimen         source.
 
Telephone Number 832-355-1000
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